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Direct Observation of Single RNA Polymerase Processing through a Single Endogenous Gene in a Living Yeast Cell
Author(s) -
Treutlein Barbara,
Michaelis Jens
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103809
Subject(s) - transcription (linguistics) , rna polymerase ii , yeast , messenger rna , microbiology and biotechnology , biology , rna , gene , gene expression , genetics , promoter , philosophy , linguistics
Rapid advances in live‐cell imaging have now enabled direct observation of the transcription of single nascent mRNA molecules from an endogenous yeast gene. A novel quantitative fluctuation analysis of fluorescently labeled mRNA revealed the kinetics of transcription initiation and the dynamics of elongation and termination (see picture; GFP=green fluorescent protein, PP7 is a bacteriophage coat protein, RNAPII=RNA polymerase II, TF=transcription factor).