Direct Observation of Single RNA Polymerase Processing through a Single Endogenous Gene in a Living Yeast Cell | Zendy

Treutlein Barbara | Zendy; Michaelis Jens | Zendy

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Direct Observation of Single RNA Polymerase Processing through a Single Endogenous Gene in a Living Yeast Cell

Author(s) -

Treutlein Barbara,

Michaelis Jens

Publication year - 2011

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201103809

Subject(s) - transcription (linguistics) , rna polymerase ii , yeast , messenger rna , microbiology and biotechnology , biology , rna , gene , gene expression , genetics , promoter , philosophy , linguistics

Rapid advances in live‐cell imaging have now enabled direct observation of the transcription of single nascent mRNA molecules from an endogenous yeast gene. A novel quantitative fluctuation analysis of fluorescently labeled mRNA revealed the kinetics of transcription initiation and the dynamics of elongation and termination (see picture; GFP=green fluorescent protein, PP7 is a bacteriophage coat protein, RNAPII=RNA polymerase II, TF=transcription factor).

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