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Covalent Protein Labeling with a Lanthanide Complex and Its Application to Photoluminescence Lifetime‐Based Multicolor Bioimaging
Author(s) -
Mizukami Shin,
Yamamoto Taku,
Yoshimura Akimasa,
Watanabe Shuji,
Kikuchi Kazuya
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103775
Subject(s) - lanthanide , luminescence , photoluminescence , nanotechnology , computer science , covalent bond , chemistry , optical imaging , computational biology , materials science , biology , optoelectronics , physics , optics , ion , organic chemistry
Better late … Lanthanide‐based time‐resolved luminescence imaging of proteins is a promising technology. A novel luminescent lanthanide probe (see picture, TPA‐Eu) for mutant β‐lactamase‐based protein labeling system (BL‐tag) is used to selectively image cell‐surface proteins (POIs) in the presence of autofluorescent background signals, thus enabling imaging with serum.