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A Direct Method for Site‐Specific Protein Acetylation
Author(s) -
Li Fupeng,
Allahverdi Abdollah,
Yang Renliang,
Lua Gavian Bing Jia,
Zhang Xiaohong,
Cao Yuan,
Korolev Nikolay,
Nordenskiöld Lars,
Liu ChuanFa
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103754
Subject(s) - acetylation , thiol , radical , lysine , chemistry , stereochemistry , combinatorial chemistry , biochemistry , amino acid , gene
Radicals at work : Radical‐mediated thiol‐ene addition of the thiol group of Cys to N ‐vinylacetamide gives acetyl‐thialysine (K S Ac), a near‐perfect mimic of acetyl‐lysine (see picture). The reaction is highly efficient with near quantitative conversion obtained in short reaction times. The generated K S Ac is functionally identical or similar to its native counterpart.