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Polarity‐Switching Electrochemical Sensor for Specific Detection of Single‐Nucleotide Mismatches
Author(s) -
Hsieh Kuangwen,
White Ryan J.,
Ferguson Brian S.,
Plaxco Kevin W.,
Xiao Yi,
Soh H. Tom
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103482
Subject(s) - polarity (international relations) , methylene blue , nucleotide , electrochemistry , chemistry , current (fluid) , redox , materials science , analytical chemistry (journal) , nanotechnology , electrode , biochemistry , physics , inorganic chemistry , gene , chromatography , thermodynamics , photocatalysis , cell , catalysis
Getting a response : A surface‐bound and redox‐modified (methylene blue) DNA probe architecture is at the heart of a sensor for detecting single‐nucleotide mismatched targets with a “polarity‐switching” response. It outputs a decreased Faradaic current when hybridized to a perfectly matched (PM) target, and an increased Faradaic current when hybridized to a single‐base mismatched (SM) target (see scheme).