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TOPP: A Novel Nitroxide‐Labeled Amino Acid for EPR Distance Measurements
Author(s) -
Stoller Sven,
Sicoli Giuseppe,
Baranova Tatiana Y.,
Bennati Marina,
Diederichsen Ulf
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103315
Subject(s) - nitroxide mediated radical polymerization , electron paramagnetic resonance , spin label , site directed spin labeling , chemistry , flexibility (engineering) , amino acid residue , amino acid , peptide , crystallography , stereochemistry , mathematics , nuclear magnetic resonance , peptide sequence , physics , biochemistry , polymer , organic chemistry , statistics , radical polymerization , gene , copolymer
A new tool : The amino acid 4‐(3,3,5,5‐tetramethyl‐2,6‐dioxo‐4‐oxylpiperazin‐1‐yl)‐ L ‐phenylglycine (TOPP), which has a rigid nitroxide spin label, can be used for EPR‐based distance measurements in peptides. The key feature of the design is the defined orientation of the nitroxide in space with respect to the peptide backbone. EPR measurements provide evidence for the low conformational flexibility of the TOPP label.