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Total Chemical Synthesis of Biologically Active Vascular Endothelial Growth Factor
Author(s) -
Mandal Kalyaneswar,
Kent Stephen B. H.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103237
Subject(s) - covalent bond , dimer , residue (chemistry) , peptide , chemistry , biological activity , chemical synthesis , vascular endothelial growth factor , chemical structure , vegf receptors , growth factor , stereochemistry , combinatorial chemistry , biochemistry , biology , in vitro , organic chemistry , cancer research , receptor
Efficient access : The 204‐residue covalent‐dimer vascular endothelial growth factor (VEGF, see picture) with full mitogenic activity was prepared from three unprotected peptide segments by one‐pot native chemical ligations. The covalent structure of the synthetic VEGF was confirmed by precise mass measurement, and the three‐dimensional structure of the synthetic protein was determined by high‐resolution X‐ray crystallography.