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Efficient Synthesis and Applications of Peptides containing Adenylylated Tyrosine Residues
Author(s) -
Smit Cornelis,
Blümer Julia,
Eerland Martijn F.,
Albers Michael F.,
Müller Matthias P.,
Goody Roger S.,
Itzen Aymelt,
Hedberg Christian
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103203
Subject(s) - legionella pneumophila , polyclonal antibodies , adenylylation , tyrosine , peptide , chemistry , gtpase , biochemistry , enzyme , biology , combinatorial chemistry , antibody , biosynthesis , genetics , bacteria
The enzyme DrrA of the human pathogen Legionella pneumophila adenylylates specifically a tyrosine of the GTPase Rab1. An efficient synthesis route using Fmoc solid phase peptide synthesis led to Tyr‐adenylylated peptides and allowed the generation of mono‐selective polyclonal antibodies against this post‐translational modification.