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On the Road towards Chemically Modified Organisms Endowed with a Genetic Firewall
Author(s) -
AcevedoRocha Carlos G.,
Budisa Nediljko
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103010
Subject(s) - genome , dna , firewall (physics) , biology , genetics , gene , physics , schwarzschild radius , classical mechanics , gravitation , charged black hole
An automated system for the continuous cultivation of cells in suspension has been used to globally change the composition of the DNA genome of E. coli. Thymine was replaced almost completely by 5‐chlorouracil through artificial evolution of cells, during which the bacterial genome accumulated hundreds of mutations under permanent conditions of proliferation and selection. The resulting 5‐chlorouracil‐adapted descendants had the ability to grow on 5‐chlorouracil.