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Nanostructured Immunosensor for Attomolar Detection of Cancer Biomarker Interleukin‐8 Using Massively Labeled Superparamagnetic Particles
Author(s) -
Munge Bernard S.,
Coffey Amy L.,
Doucette Jaimee M.,
Somba Brian K.,
Malhotra Ruchika,
Patel Vyomesh,
Gutkind J. Silvio,
Rusling James F.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201102941
Subject(s) - superparamagnetism , horseradish peroxidase , conjugate , chemistry , biomarker , nanotechnology , nanoparticle , magnetic nanoparticles , materials science , organic chemistry , magnetization , biochemistry , enzyme , physics , quantum mechanics , magnetic field , mathematical analysis , mathematics
A sensitive issue : Superparamagnetic particle–antibody conjugates enable detection of interleukin‐8 (IL‐8) in serum at a level of 1 fg mL −1 (ca. 100 a M ). Ultrahigh sensitivity is facilitated by a nanostructured sensor platform coupled with secondary antibody–magnetic bead–horseradish peroxidase conjugate (Ab 2 –MB–HRP) with about 500 000 HRP labels (see picture).