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Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases
Author(s) -
Kielkowski Pavel,
MacíčkováCahová Hana,
Pohl Radek,
Hocek Michal
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201102898
Subject(s) - restriction enzyme , dna , cleavage (geology) , dna polymerase , polymerase , primer (cosmetics) , chemistry , microbiology and biotechnology , primer extension , biology , biochemistry , base sequence , paleontology , organic chemistry , fracture (geology)
Restricted access to DNA : (Triethylsilyl)ethynyl‐modified 7‐deaza‐dATP is readily incorporated into DNA by primer extension or polymerase chain reaction using KOD XL polymerase. The silylethynyl‐ protected DNA resists the cleavage by restriction endonucleases (REs), such as RsaI (see scheme), but after it is treated with NH 3 , the resulting deprotected DNA is fully cleavable by the REs.