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Dynamic Monitoring of Newly Synthesized Proteomes: Up‐Regulation of Myristoylated Protein Kinase A During Butyric Acid Induced Apoptosis
Author(s) -
Liu Kai,
Yang PengYu,
Na Zhenkun,
Yao Shao Q.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201102542
Subject(s) - butyric acid , apoptosis , proteome , activator (genetics) , jurkat cells , kinase , christian ministry , methionine , chemistry , microbiology and biotechnology , computational biology , computer science , biochemistry , amino acid , biology , gene , genetics , t cell , philosophy , immune system , theology
Doubly charged : A double metabolic incorporation approach capable of proteome‐wide profiling of post‐translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up‐regulation of myristoylated PKA protein is necessary for the occurrence of butyric acid induced apoptosis in Jurkat cells.