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Expanded Click Conjugation of Recombinant Proteins with Ubiquitin‐Like Modifiers Reveals Altered Substrate Preference of SUMO2‐Modified Ubc9
Author(s) -
Sommer Stefanie,
Weikart Nadine D.,
Brockmeyer Andreas,
Janning Petra,
Mootz Henning D.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201102531
Subject(s) - mutagenesis , recombinant dna , preference , computational biology , chemistry , ubiquitin , click chemistry , substrate (aquarium) , protein engineering , biochemistry , combinatorial chemistry , computer science , genetics , biology , mutation , enzyme , gene , ecology , economics , microeconomics
Wrestling with SUMO : The chemical conjugation of proteins with small ubiquitin‐like modifiers (SUMO) can be achieved by a copper(I)‐catalyzed cycloaddition and unnatural amino acid mutagenesis (see scheme). This approach overcomes previous restrictions related to the primary sequence of proteins and coupling conditions. Moreover, biochemical data suggests that this triazole linkage presents the modifier in a proper distance and orientation relative to the target protein.