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Biosynthesis of Himastatin: Assembly Line and Characterization of Three Cytochrome P450 Enzymes Involved in the Post‐tailoring Oxidative Steps
Author(s) -
Ma Junying,
Wang Zhongwen,
Huang Hongbo,
Luo Minghe,
Zuo Dianguang,
Wang Bo,
Sun Aijun,
Cheng YiQiang,
Zhang Changsheng,
Ju Jianhua
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201102305
Subject(s) - complementation , oxidative phosphorylation , cytochrome p450 , enzyme , biochemistry , mutant , oxidative enzyme , biosynthesis , gene , chemistry , assembly line , oxidoreductase , biology , mechanical engineering , engineering
It's only natural : The biosynthetic pathway of himastatin from Streptomyces himastatinicus featuring unusual domain organizations is elucidated by genome scanning, generation of in‐frame gene deletion mutants, and complementation experiments. Three cytochrome P450s that catalyze novel post‐tailoring oxidative reactions (see picture) are characterized.