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Single Gold Nanoparticles as Real‐Time Optical Probes for the Detection of NADH‐Dependent Intracellular Metabolic Enzymatic Pathways
Author(s) -
Zhang Lei,
Li Yang,
Li DaWei,
Jing Chao,
Chen Xiaoyuan,
Lv Min,
Huang Qing,
Long YiTao,
Willner Itamar
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201102151
Subject(s) - surface plasmon resonance , biosensor , colloidal gold , chemistry , nanoparticle , rayleigh scattering , intracellular , dynamic light scattering , spectroscopy , biophysics , plasmon , nanotechnology , materials science , analytical chemistry (journal) , biochemistry , optoelectronics , biology , optics , chromatography , physics , quantum mechanics
A coat of many colors : Plasmon resonance Rayleigh scattering (PRRS) spectroscopy is applied to the in‐situ monitoring of the NADH‐dependent growth of Au@Cu core–shell nanoparticles (NPs). The scattering signal of the AuNPs is red shifted when they are coated with Cu (see scheme). In conjunction with dark‐field microscopy, a colorimetric NADH‐dependent biosensor was constructed and tested to screen an anticancer drug affecting cell NADH metabolism.