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Genetically Programmed In Vivo Packaging of Protein Cargo and Its Controlled Release from Bacteriophage P22
Author(s) -
O'Neil Alison,
Reichhardt Courtney,
Johnson Benjamin,
Prevelige Peter E.,
Douglas Trevor
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201102036
Subject(s) - capsid , bacteriophage , green fluorescent protein , chemistry , in vivo , scaffold , microbiology and biotechnology , biophysics , virus , nanotechnology , materials science , biology , virology , biochemistry , gene , escherichia coli , computer science , database
Packed and ready to go : A scaffold protein (SP) aids the assembly of Salmonella typhimuriam bacteriophage P22 into a capsid, with encapsulation of the SP. This natural process was exploited by using an engineered molecular system to fuse a fluorescent protein cargo (green in the picture) to a portion of the SP (yellow), which templated accurate spontaneous assembly. Heating of the capsids and treatment with thrombin released the SP but not the cargo.