Premium
Rapid Identification of Noncanonical RNA Structure Elements by Direct Detection of OH⋅⋅⋅OP, NH⋅⋅⋅OP, and NH 2 ⋅⋅⋅OP Hydrogen Bonds in Solution NMR Spectroscopy
Author(s) -
DuchardtFerner Elke,
Ferner Jan,
Wöhnert Jens
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101743
Subject(s) - hydrogen bond , rna , chemistry , crystallography , nuclear magnetic resonance spectroscopy , spectroscopy , hydrogen , scalar (mathematics) , identification (biology) , stereochemistry , molecule , physics , mathematics , organic chemistry , quantum mechanics , biochemistry , biology , botany , gene , geometry
Hydrogen bonds involving phosphate groups as acceptors only occur in noncanonical structure elements of RNAs and are important for their stable folding. These hydrogen bonds can be directly assigned by H,P correlation experiments in solution NMR spectroscopy owing to the presence of sizeable 2h J HP scalar couplings across the hydrogen bond. These experiments will provide valuable information to speed up the determination of complex RNA structures.