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Small‐Molecule‐Based Nanoassemblies as Inducible Nanoprobes for Monitoring Dynamic Molecular Interactions Inside Live Cells
Author(s) -
Lee Sangkyu,
Lee Kyoung Hu,
Ha JaeSeok,
Lee SeungGoo,
Kim Tae K.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101467
Subject(s) - small molecule , intracellular , nanotechnology , biophysics , nanosensor , nanoparticle , protein–protein interaction , chemistry , computational biology , biology , microbiology and biotechnology , materials science , biochemistry
Seeing into cells : Genetically engineered, labeled ferritin is used to form biological nanoparticles displaying bait (B) and prey (P) molecules inside cells (see picture). Specific bait–prey interactions induce the interconnected assembly of nanoparticles into nanoclusters on a timescale of seconds. This phenotypic readout with a small molecule as an inducible switch was used to directly visualize dynamic molecular interactions within living cells.