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Highly Selective Fluorescent Probe for Vicinal‐Dithiol‐Containing Proteins and In Situ Imaging in Living Cells
Author(s) -
Huang Chusen,
Yin Qin,
Zhu Weiping,
Yang Yi,
Wang Xin,
Qian Xuhong,
Xu Yufang
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101317
Subject(s) - vicinal , fluorescence , in situ , chemistry , computational biology , computer science , biology , biochemistry , optics , physics , organic chemistry
It pays to be direct : In a rapid and specific approach to the detection of vicinal‐dithiol‐containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.