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Membrane Anchored Immunostimulatory Oligonucleotides for In Vivo Cell Modification and Localized Immunotherapy
Author(s) -
Liu Haipeng,
Kwong Brandon,
Irvine Darrell J.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101266
Subject(s) - oligonucleotide , nuclease , in vivo , chemistry , dna , membrane , cpg site , rna , cell , covalent bond , microbiology and biotechnology , biophysics , biochemistry , biology , gene , dna methylation , gene expression , organic chemistry
Amphiphilic oligonucleotides synthesized by covalent conjugation between a hydrophobic diacyllipid tail and chemically stabilized RNA or DNA oligonucleotides can directly label tumor cells on injection into solid tumors. In a murine melanoma tumor model, cell membrane‐anchored CpG ODNs with a nuclease‐resistant phophorothioate backbone (row a) exhibited significantly enhanced immunostimulatory activity compared to soluble CpG (row b).

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