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A Microbiological–Chemical Strategy to Produce Chondroitin Sulfate A,C
Author(s) -
Bedini Emiliano,
De Castro Cristina,
De Rosa Mario,
Di Nola Annalida,
Iadonisi Alfonso,
Restaino Odile F.,
Schiraldi Chiara,
Parrilli Michelangelo
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101142
Subject(s) - chondroitin sulfate , sulfation , yield (engineering) , fermentation , chondroitin , computer science , food science , chemistry , regioselectivity , microbiology and biotechnology , combinatorial chemistry , biochemistry , biology , glycosaminoglycan , materials science , metallurgy , catalysis
No pigs, no cows needed : Chondroitin sulfate for pharmacological uses is obtained from animal sources in low abundance and with a laborious downstream purification. It has now been synthesized for the first time by an innovative microbiological–chemical approach consisting of chondroitin production from E. coli K4 fed‐batch fermentation and its subsequent regioselective sulfation in five steps and 61 % overall yield (see scheme).