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Lanthanide‐Coded Protease‐Specific Peptide–Nanoparticle Probes for a Label‐Free Multiplex Protease Assay Using Element Mass Spectrometry: A Proof‐of‐Concept Study
Author(s) -
Yan Xiaowen,
Yang Limin,
Wang Qiuquan
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101087
Subject(s) - multiplex , protease , peptide , mass spectrometry , chemistry , inductively coupled plasma mass spectrometry , nanoparticle , lanthanide , chromatography , computational biology , combinatorial chemistry , nanotechnology , biochemistry , bioinformatics , materials science , biology , enzyme , organic chemistry , ion
Coded messages : Lanthanide (Ln) coded protease‐specific peptide–nanoparticle (NPs) conjugates act as probes for a multiplex protease assay (MPA) using inductively coupled plasma mass spectrometry (ICP‐MS; see picture). MPA can be achieved by centrifugal separation of the protease‐cleaved and uncleaved probes and subsequent determination of the released Ln tags.