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Detection of a Protein Conformational Equilibrium by Electrospray Ionisation‐Ion Mobility‐Mass Spectrometry
Author(s) -
Jenner Matthew,
Ellis Jacqueline,
Huang WeiCheng,
Lloyd Raven Emma,
Roberts Gordon C. K.,
Oldham Neil J.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101077
Subject(s) - ion mobility spectrometry , mass spectrometry , chemistry , electrospray ionization , ion , electrospray , abundance (ecology) , ionic bonding , ionic strength , analytical chemistry (journal) , dynamic equilibrium , chromatography , organic chemistry , fishery , aqueous solution , biology
Conforming to convention : Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the ionic strength of the solution from which they are electrosprayed.