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Reliable and Efficient Procedures for the Conjugation of Biomolecules through Huisgen Azide–Alkyne Cycloadditions
Author(s) -
Lallana Enrique,
Riguera Ricardo,
FernandezMegia Eduardo
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201101019
Subject(s) - cycloaddition , azide , biomolecule , chemistry , alkyne , combinatorial chemistry , bioorthogonal chemistry , click chemistry , nucleic acid , surface modification , organic chemistry , catalysis , biochemistry
The Cu I ‐catalyzed azide–alkyne cycloaddition (CuAAC) has been established as a powerful coupling technology for the conjugation of proteins, nucleic acids, and polysaccharides. Nevertheless, several shortcomings related to the presence of Cu, mainly oxidative degradation by reactive oxygen species and sample contamination by Cu, have been pointed out. This Minireview discusses key aspects found in the development of the efficient and benign functionalization of biomacromolecules through CuAAC, as well as the Cu‐free strain‐promoted azide–alkyne cycloaddition (SPAAC).