Hypersensitive Response to Over‐reactive Cysteines

Finding the active cysteine in a hay stack : In the search for active proteins and active‐site peptides in a proteome, techniques such as quantitative proteomics and protein profiling with activity‐based probes (ABPs; see picture) are commonly used. A recently developed proteomics technique combines these approaches to globally identify reactive cysteines, and possibly other amino acids, in a complex proteome.