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One‐Pot Dual‐Labeling of a Protein by Two Chemoselective Reactions
Author(s) -
Yi Long,
Sun Hongyan,
Itzen Aymelt,
Triola Gemma,
Waldmann Herbert,
Goody Roger S.,
Wu YaoWen
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201100840
Subject(s) - rab , gtpase , computer science , dual (grammatical number) , folding (dsp implementation) , computational biology , information retrieval , chemistry , biochemistry , biology , linguistics , engineering , philosophy , electrical engineering
Dual system for proteins : The site‐specific two‐color labeling of a Rab GTPase was achieved in a one‐pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein–protein interaction studies.