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Sensitive and Selective Bacterial Detection Using Tetracysteine‐Tagged Phages in Conjunction with Biarsenical Dye
Author(s) -
Wu Lina,
Huang Tingting,
Yang Lingling,
Pan Jianbo,
Zhu Shaobin,
Yan Xiaomei
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201100334
Subject(s) - flow cytometry , fluorescence microscope , fluorescence , capsid , bacteria , chemistry , microbiology and biotechnology , biophysics , bacteriophage , microscopy , membrane , biology , biochemistry , escherichia coli , gene , genetics , physics , quantum mechanics , optics
A host‐specific phage is genetically modified to tag a tetracysteine (TC) residue to its capsid protein. Upon bacterial infection, the TC tag is expressed on the surface of progeny virions and stained by membrane‐permeant biarsenical dye, which results in fluorescence enhancement (see picture). The labeled bacteria are detectable by flow cytometry and fluorescence microscopy.