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Time‐Resolved FRET Biosensor Based on Amine‐Functionalized Lanthanide‐Doped NaYF 4 Nanocrystals
Author(s) -
Tu Datao,
Liu Liqin,
Ju Qiang,
Liu Yongsheng,
Zhu Haomiao,
Li Renfu,
Chen Xueyuan
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201100303
Subject(s) - förster resonance energy transfer , biosensor , energy transfer , nanocrystal , biotinylation , biomolecule , lanthanide , nanotechnology , fluorescence , excitation , chemistry , isothiocyanate , materials science , physics , optics , biochemistry , organic chemistry , ion , quantum mechanics , molecular physics
Background elimination and improved sensitivity were achieved by time‐resolved (TR) detection with a FRET biosensor for traces of biomolecules such as avidin at concentrations down to 4.8 n M . As shown in the picture, UV excitation of biotinylated NaYF 4 :Ce/Tb nanocrystals triggers energy transfer to fluorescein isothiocyanate (FITC), whose long‐lived emission due to FRET can be distinguished from the short‐lived background from direct excitation.