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Simultaneous Concentration and Separation of Proteins in a Nanochannel
Author(s) -
Inglis David W.,
Goldys Ewa M.,
Calander Nils P.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201100236
Subject(s) - chemistry , concentration gradient , chromatography , electrophoresis , separation (statistics) , salt (chemistry) , conductivity , matrix (chemical analysis) , chemical physics , analytical chemistry (journal) , computer science , organic chemistry , machine learning
Balancing the forces : Conductivity gradient focusing in a nanochannel achieves simultaneous separation and concentration of proteins without a temperature gradient, membrane, matrix, ampholytes, or external pump. The electrophoretic force (see picture; red arrow) that dominates at the low‐salt side (−) balances the electro‐osmotic force (blue) at the high‐salt side (+).