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Chemical and Chemoenzymatic Syntheses of Bacillithiol: A Unique Low‐Molecular‐Weight Thiol amongst Low G + C Gram‐Positive Bacteria
Author(s) -
Sharma Sunil V.,
Jothivasan Vishnu K.,
Newton Gerald L.,
Upton Heather,
Wakabayashi Judy I.,
Kane Melissa G.,
Roberts Alexandra A.,
Rawat Mamta,
La Clair James J.,
Hamilton Chris J.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201100196
Subject(s) - thiol , chemistry , cysteine , biochemistry , stereochemistry , bacteria , substrate (aquarium) , transferase , enzyme , biology , genetics , ecology
The full monty : The recently discovered thiol cofactor bacillithiol (BSH), its biosynthetic precursors, and its symmetrical disulfide are prepared in two ways. The fosfomycin resistance protein (FosB) is shown to be a BSH‐utilizing enzyme. It displays bacillithiol‐ S ‐transferase activity with a strong preference for BSH over L ‐cysteine as its thiol substrate.