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Quantum‐Dot‐Based FRET Detection of Histone Acetyltransferase Activity
Author(s) -
Ghadiali James E.,
Lowe Stuart B.,
Stevens Molly M.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201008263
Subject(s) - förster resonance energy transfer , quantum dot , histone acetyltransferase , histone , chemistry , optoelectronics , physics , computational biology , biophysics , biology , quantum mechanics , biochemistry , fluorescence , gene
Straightforward and label‐free : A simple method for the detection of histone‐modifying enzymes is based on quantum dot (QD) Förster resonance energy transfer (FRET) donors. Histone acetyltransferase activity can be measured via the enzyme‐dependent assembly of a quantum dot/peptide immunocomplex (see picture; His=histidine), which can be conducted in a homogeneous reaction without the need for complicated surface derivatization procedures.