Premium
Glycan Structure of a High‐Mannose Glycoprotein from Raman Optical Activity
Author(s) -
Johannessen Christian,
Pendrill Robert,
Widmalm Göran,
Hecht Lutz,
Barron Laurence D.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201008258
Subject(s) - mannose , glycan , glycoprotein , chemistry , raman spectroscopy , biochemistry , invertase , enzyme , physics , optics
A revealing signature : The glycan structure of intact yeast external invertase, a high‐mannose glycoprotein used as biocatalyst, was investigated by using Raman optical activity (ROA) spectroscopy. The conformational preferences present in mannose‐containing di‐ and trisaccharides were found to be preserved in the glycan chains, with secondary polpeptide backbone structure suppressed.