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Genetically Encoded Copper‐Free Click Chemistry
Author(s) -
Plass Tilman,
Milles Sigrid,
Koehler Christine,
Schultz Carsten,
Lemke Edward A.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201008178
Subject(s) - click chemistry , chemistry , azide , combinatorial chemistry , genetic code , in vitro , transfer rna , biochemistry , gene , rna , organic chemistry
A bright click of life : Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site‐specifically in proteins allowing efficient labeling with fluorogenic azide‐bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme).