Genetically Encoded Copper‐Free Click Chemistry | Zendy

Plass Tilman | Zendy; Milles Sigrid | Zendy; Koehler Christine | Zendy; Schultz Carsten | Zendy; Lemke Edward A. | Zendy

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Genetically Encoded Copper‐Free Click Chemistry

Author(s) -

Plass Tilman,

Milles Sigrid,

Koehler Christine,

Schultz Carsten,

Lemke Edward A.

Publication year - 2011

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201008178

Subject(s) - click chemistry , chemistry , azide , combinatorial chemistry , genetic code , in vitro , transfer rna , biochemistry , gene , rna , organic chemistry

A bright click of life : Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site‐specifically in proteins allowing efficient labeling with fluorogenic azide‐bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme).

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