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Artificial Transfer Hydrogenases for the Enantioselective Reduction of Cyclic Imines
Author(s) -
Dürrenberger Marc,
Heinisch Tillmann,
Wilson Yvonne M.,
Rossel Thibaud,
Nogueira Elisa,
Knörr Livia,
Mutschler Annette,
Kersten Karoline,
Zimbron Malcolm Jeremy,
Pierron Julien,
Schirmer Tilman,
Ward Thomas R.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007820
Subject(s) - enantioselective synthesis , transfer hydrogenation , streptavidin , imine , combinatorial chemistry , chemistry , mutagenesis , lysine , stereochemistry , computer science , biochemistry , catalysis , mutation , biotin , amino acid , ruthenium , gene
Man‐made activity : Introduction of a biotinylated iridium piano stool complex within streptavidin affords an artificial imine reductase (see scheme). Saturation mutagenesis allowed optimization of the activity and the enantioselectivity of this metalloenzyme, and its X‐ray structure suggests that a nearby lysine residue acts as a proton source during the transfer hydrogenation.