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Solid‐State NMR Spectroscopic Analysis of the Ca 2+ ‐Dependent Mannose Binding of Pradimicin A
Author(s) -
Nakagawa Yu,
Masuda Yuichi,
Yamada Keita,
Doi Takashi,
Takegoshi K.,
Igarashi Yasuhiro,
Ito Yukishige
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007775
Subject(s) - mannose , nuclear magnetic resonance spectroscopy , chemistry , solid state nuclear magnetic resonance , spectroscopy , stereochemistry , nuclear magnetic resonance , physics , biochemistry , quantum mechanics
Aggregation facilitates analysis : The Ca 2+ ‐dependent mannose (Man) binding of the nonpeptidic carbohydrate binder pradimicin A (PRM‐A) was investigated in the solid state. The use of PRM‐A aggregates eliminated problems associated with the three‐component equilibrium. A combination of 113 Cd NMR spectroscopy and 2D dipolar‐assisted rotational resonance revealed the mannose‐binding site of PRM‐A and the crucial role of the Ca 2+ ion (see binding model).