Solid‐State NMR Spectroscopic Analysis of the Ca 2+ ‐Dependent Mannose Binding of Pradimicin A | Zendy

Nakagawa Yu | Zendy; Masuda Yuichi | Zendy; Yamada Keita | Zendy; Doi Takashi | Zendy; Takegoshi K. | Zendy; Igarashi Yasuhiro | Zendy; Ito Yukishige | Zendy

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Solid‐State NMR Spectroscopic Analysis of the Ca 2+ ‐Dependent Mannose Binding of Pradimicin A

Author(s) -

Nakagawa Yu,

Masuda Yuichi,

Yamada Keita,

Doi Takashi,

Takegoshi K.,

Igarashi Yasuhiro,

Ito Yukishige

Publication year - 2011

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201007775

Subject(s) - mannose , nuclear magnetic resonance spectroscopy , chemistry , solid state nuclear magnetic resonance , spectroscopy , stereochemistry , nuclear magnetic resonance , physics , biochemistry , quantum mechanics

Aggregation facilitates analysis : The Ca 2+ ‐dependent mannose (Man) binding of the nonpeptidic carbohydrate binder pradimicin A (PRM‐A) was investigated in the solid state. The use of PRM‐A aggregates eliminated problems associated with the three‐component equilibrium. A combination of 113 Cd NMR spectroscopy and 2D dipolar‐assisted rotational resonance revealed the mannose‐binding site of PRM‐A and the crucial role of the Ca 2+ ion (see binding model).

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