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Optimal Codon Choice Can Improve the Efficiency and Fidelity of N ‐Methyl Amino Acid Incorporation into Peptides by In‐Vitro Translation
Author(s) -
Subtelny Alexander O.,
Hartman Matthew C. T.,
Szostak Jack W.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007686
Subject(s) - translation (biology) , amino acid , in vitro , peptide , codon usage bias , computational biology , chemistry , computer science , fidelity , combinatorial chemistry , biochemistry , biology , gene , messenger rna , genome , telecommunications
May the best codon win : N ‐methyl amino acids ( N ‐Me AAs) are important modifications found in several nonribosomal peptide therapeutics. Previously, it was shown that three N ‐Me AAs are efficiently incorporated into peptides using a cell‐free in‐vitro translation system. Now, the incorporation of these N ‐Me AAs has been optimized by varying the template codon.