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An Effective Minor Groove Binder as a Red Fluorescent Marker for Live‐Cell DNA Imaging and Quantification
Author(s) -
Peng Xiaojun,
Wu Tong,
Fan Jiangli,
Wang Jingyun,
Zhang Si,
Song Fengling,
Sun Shiguo
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007386
Subject(s) - minor groove , dna , fluorescence , biophysics , groove (engineering) , chemistry , live cell imaging , in vivo , cell , materials science , biochemistry , biology , optics , genetics , physics , metallurgy
Seeing red : DEAB‐TO‐3 shows a large fluorescence enhancement upon binding to native DNA and a distinct selectivity for double‐stranded DNA over total RNA. As a red fluorescent live‐cell‐permeant DNA minor groove binder, DEAB‐TO‐3 is promising for highly sensitive DNA detection in vitro and nucleus‐specific imaging and DNA quantification in vivo (see picture).