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An Iminodiacetic Acid Based Lanthanide Binding Tag for Paramagnetic Exchange NMR Spectroscopy
Author(s) -
Swarbrick James D.,
Ung Phuc,
Chhabra Sandeep,
Graham Bim
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007221
Subject(s) - iminodiacetic acid , paramagnetism , lanthanide , spectroscopy , nuclear magnetic resonance spectroscopy , chemistry , electron paramagnetic resonance spectroscopy , metal , crystallography , nuclear magnetic resonance , stereochemistry , physics , condensed matter physics , organic chemistry , electron paramagnetic resonance , ion , quantum mechanics
All the way with IDA! Attachment of iminodiacetic acid (IDA) to a protein helix creates a rigid lanthanide binding site that can be exploited for paramagnetic NMR spectroscopy (see picture). Pseudo‐contact shifts (PCSs) larger than 8 ppm are achievable with the tag, and metal exchange is sufficiently fast to enable signal assignment by 15 N z exchange spectroscopy, eliminating the need for an initial protein model.