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Superresolution Imaging of Albumin‐Conjugated Fluorescent Nanodiamonds in Cells by Stimulated Emission Depletion
Author(s) -
Tzeng YanKai,
Faklaris Orestis,
Chang BeMing,
Kuo Yung,
Hsu JuiHung,
Chang HuanCheng
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007215
Subject(s) - sted microscopy , fluorescence , conjugated system , confocal , bovine serum albumin , superresolution , chemistry , fluorescence lifetime imaging microscopy , biophysics , optics , biochemistry , biology , polymer , image (mathematics) , organic chemistry , physics , artificial intelligence , computer science
A bunch of loners : Fluorescent nanodiamonds (FNDs) noncovalently conjugated with bovine serum albumin (BSA) or α‐lactalbumin exhibited good dispersibility in a buffer with only minor or no agglomeration. They are useful as photostable fluorescent markers in cells for superresolution imaging by STED (see confocal fluorescence image of an FND‐labeled cell (left) and STED image of single BSA‐conjugated FNDs (right)).