Premium
Quantification of Protein Sulfenic Acid Modifications Using Isotope‐Coded Dimedone and Iododimedone
Author(s) -
Seo Young Ho,
Carroll Kate S.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007175
Subject(s) - sulfenic acid , dimedone , chemistry , proteomics , peptide , quantitative proteomics , stable isotope labeling by amino acids in cell culture , biochemistry , enzyme , cysteine , catalysis , gene
Quantitative proteomics : The new technique mentioned in the title—in short, ICDID—enables quantification of sulfenic acid modifications in proteins (see picture). The approach permits S‐hydroxylation site occupancy to be monitored at individual cysteines within a single protein and is compatible with peptide‐based proteomic strategies.