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Methyltransferase‐Directed Derivatization of 5‐Hydroxymethylcytosine in DNA
Author(s) -
Liutkevičiūtė Zita,
Kriukienė Edita,
Grigaitytė Indrė,
Masevičius Viktoras,
Klimašauskas Saulius
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007169
Subject(s) - 5 hydroxymethylcytosine , cytosine , dna methylation , methylation , derivatization , dna , methyltransferase , chemistry , nucleobase , epigenetics , biochemistry , computational biology , biology , gene , mass spectrometry , chromatography , gene expression
Sequence‐specific derivatization : Enzymatic methylation of cytosine in DNA is part of an epigenetic regulatory network in vertebrates. In the absence of the methylation cofactor S ‐adenosyl‐ L ‐methionine, bacterial cytosine‐5 methyltransferases can catalyze the condensation of aliphatic thiols and selenols with 5‐hydroxymethylcytosine, a recently discovered nucleobase in mammalian DNA, to yield 5‐chalcogenomethyl derivatives (see scheme).