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Membrane‐Bound Stable Glycosyltransferases: Highly Oriented Protein Immobilization by a C‐Terminal Cationic Amphipathic Peptide
Author(s) -
Naruchi Kentaro,
Nishimura ShinIchiro
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201007153
Subject(s) - glycosyltransferase , amphiphile , fucosyltransferase , cationic polymerization , peptide , membrane , chemistry , enzyme , biochemistry , recombinant dna , copolymer , gene , organic chemistry , polymer
Turning tail : The specific functions of C‐terminal cationic amphipathic peptide in the formation of bacterial membrane‐bound glycosyltransferases have been demonstrated. This mechanism was applied to a general concept that allows highly oriented immobilization of enzymes on membrane‐mimetic solid surfaces, such as recombinant full‐length H. pylori α1,3‐fucosyltransferase on a magnetic bead (see picture).