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In Vivo Solid‐Phase Microextraction in Metabolomics: Opportunities for the Direct Investigation of Biological Systems
Author(s) -
Vuckovic Dajana,
Risticevic Sanja,
Pawliszyn Janusz
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006896
Subject(s) - solid phase microextraction , metabolomics , context (archaeology) , metabolome , organism , computer science , biochemical engineering , computational biology , chemistry , biology , chromatography , mass spectrometry , gas chromatography–mass spectrometry , engineering , paleontology
Sample preparation has a strong impact on the quality of metabolomics studies. The use of solid‐phase microextraction (SPME), particularly its in vivo format, enables the capture of a more representative metabolome and presents opportunities to detect low‐abundance, short‐lived, and/or unstable species not easily captured by traditional methods. The technique is ideally suited for temporal, spatial, and longitudinal studies of the same living system, as well as multicompartmental studies of the same organism. SPME is useful for the investigation of biological systems ranging in complexity from cells to mammalian tissues. Selected examples are highlighted in this Minireview in order to place the technique within the context of conventional methods of sample preparation for metabolomics.