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Direct Genetic Analysis of Ten Cancer Cells: Tuning Sensor Structure and Molecular Probe Design for Efficient mRNA Capture
Author(s) -
Vasilyeva Elizaveta,
Lam Brian,
Fang Zhichao,
Minden Mark D.,
Sargent Edward H.,
Kelley Shana O.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006793
Subject(s) - nucleic acid , cancer , messenger rna , chip , solubility , nanotechnology , cancer cell , computational biology , leukemia , chemistry , biology , microbiology and biotechnology , materials science , combinatorial chemistry , cancer research , biochemistry , computer science , genetics , gene , telecommunications
A chip‐based platform is reported that is able to detect as few as 10 cancer cells. By developing sub‐milliscale sensors that are able to capture slow moving biological targets with high efficiency (see picture; scale bar 50 μm), cancer‐specific sequences were detected in crude lysates of leukemia cells. This achievement relied on the development of a new type of molecular probe that improves the solubility and performance of neutral nucleic acids.