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SEIRA Spectroscopy of the Electrochemical Activation of an Immobilized [NiFe] Hydrogenase under Turnover and Non‐Turnover Conditions
Author(s) -
Millo Diego,
Hildebrandt Peter,
Pandelia MariaEirini,
Lubitz Wolfgang,
Zebger Ingo
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006646
Subject(s) - hydrogenase , enzyme , active site , spectroscopy , infrared spectroscopy , electrochemistry , redox , chemistry , titration , function (biology) , near infrared spectroscopy , combinatorial chemistry , biochemistry , electrode , inorganic chemistry , biology , organic chemistry , physics , neuroscience , evolutionary biology , quantum mechanics
Effective and unaffected : An [NiFe] hydrogenase was immobilized on Au electrodes. Surface‐enhanced infrared absorption (SEIRA) spectroscopy monitored structural changes of the active site as a function of the applied potential. The redox titration of the enzyme gave a formal potential ( E 1/2 ) similar to that obtained for the enzyme in solution, demonstrating that the structure of the active site is not affected by the immobilization.