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13 C Direct‐Detection Biomolecular NMR Spectroscopy in Living Cells
Author(s) -
Bertini Ivano,
Felli Isabella C.,
Gonnelli Leonardo,
Kumar M. Vasantha,
Pierattelli Roberta
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006636
Subject(s) - heteronuclear molecule , nuclear magnetic resonance spectroscopy , chemistry , heteronuclear single quantum coherence spectroscopy , spectroscopy , carbon 13 nmr , crystallography , nuclear magnetic resonance , stereochemistry , physics , quantum mechanics
A direct response : Exclusively heteronuclear 13 C direct‐detection 2D NMR experiments are performed on 13 C, 15 N‐enriched proteins in E. coli cells. Unfolded proteins or protein fragments provide well‐resolved carbonyl detection (see picture), whereas folded proteins or structured motifs do not give any detectable spectrum.