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Unique Gene‐Silencing and Structural Properties of 2′‐Fluoro‐Modified siRNAs
Author(s) -
Manoharan Muthiah,
Akinc Akin,
Pandey Rajendra K.,
Qin June,
Hadwiger Philipp,
John Matthias,
Mills Kathy,
Charisse Klaus,
Maier Martin A.,
Nechev Lubomir,
Greene Emily M.,
Pallan Pradeep S.,
Rozners Eriks,
Rajeev Kallanthottathil G.,
Egli Martin
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006519
Subject(s) - small interfering rna , gene silencing , nuclease , computational biology , gene , trans acting sirna , chemistry , rna , biology , biochemistry
With little or no negative impact on the activity of small interfering RNAs (siRNAs), regardless of the number of modifications or the positions within the strand, the 2′‐deoxy‐2′‐fluoro (2′‐F) modification is unique. Furthermore, the 2′‐F‐modified siRNA (see crystal structure) was thermodynamically more stable and more nuclease‐resistant than the parent siRNA, and produced no immunostimulatory response.