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Chemical Dissection of Protein Translocation through the Anthrax Toxin Pore
Author(s) -
Pentelute Brad L.,
Sharma Onkar,
Collier R. John
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006460
Subject(s) - anthrax toxin , chromosomal translocation , toxin , chemistry , sequence (biology) , amino acid , cytoplasm , biophysics , biochemistry , biology , fusion protein , recombinant dna , gene
Through the pore : The N‐terminal domain of the lethal factor of anthrax toxin was modified to probe protein translocation through the toxin pore. Replacing acidic residues with cysteic acid inhibited translocation, whereas introduction of D ‐amino acids or an alternating Lys–Glu sequence had no effect. The findings demonstrate independence of translocation from stereospecificity and strict sequence, and dependence on the charge state of acidic residues.