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Identification of Drug Targets In Vitro and in Living Cells by Soluble‐Nanopolymer‐Based Proteomics
Author(s) -
Hu Lianghai,
Iliuk Anton,
Galan Jacob,
Hans Michael,
Tao W. Andy
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006459
Subject(s) - lysis , in vitro , drug , computational biology , identification (biology) , proteomics , chemistry , mass spectrometry , conjugated system , computer science , biochemistry , biology , pharmacology , chromatography , gene , botany , organic chemistry , polymer
Getting in where the action is : Drug‐conjugated dendrimers were used in combination with mass spectrometric analysis to identify drug targets in vitro and in living cells. In this proteomic strategy, the drug‐conjugated nanopolymer is incubated with cells to ensure efficient delivery (see picture), and the cells are then lysed. Proteins bound to the drug are isolated on a solid support and identified by mass spectrometry.