Premium
Design of a Three‐Helix Bundle Capable of Binding Heavy Metals in a Triscysteine Environment
Author(s) -
Chakraborty Saumen,
Yudenfreund Kravitz Joslyn,
Thulstrup Peter W.,
Hemmingsen Lars,
DeGrado William F.,
Pecoraro Vincent L.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006413
Subject(s) - library science , chemistry , computer science
A dream becomes reality : Until now, the engineering of novel metal‐binding sites into a three‐helix bundle has been an unrealized dream. By using an existing three‐helix bundle as a template, a 3‐Cys metal‐binding site was generated at the C‐terminal end by rational design to yield a well‐folded and stable construct that encapsulates the heavy metals Hg II , Cd II , and Pb II with high affinity and predefined coordination geometry (see picture).