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MALDI‐TOF Mass Spectrometric Analysis of Enzyme Activity and Lectin Trapping on an Array of N‐Glycans
Author(s) -
SanchezRuiz Antonio,
Serna Sonia,
Ruiz Nerea,
MartinLomas Manuel,
Reichardt NielsChristian
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006304
Subject(s) - lectin , glycan , chemistry , galactosyltransferase , alkyl , enzyme , trapping , monolayer , biochemistry , chromatography , combinatorial chemistry , organic chemistry , glycoprotein , biology , ecology
Active arrays : Complex lipid‐tagged oligosaccharides, including large multiantennary species, can be efficiently immobilized on self‐assembled monolayers of alkyl mercaptans (see picture). These arrays can be used to follow the action of a galactosyltransferase (GalT) and a hydrolase. The utility of the system for the selective trapping and identification of a lectin from a complex mixture was also demonstrated.